Organic microstructures are well known for their resonator properties, which bring about whispering gallery mode (WGM) excitation. Here we report on experimental evidence of the WGM in the two-photon fluorescence (TPF) of DCM dye microspheres made using the selfassembly method. The WGM excitation accompanying the overall TPF in the spectral range from 530 ÷ 640 nm demonstrated a quality factor of approximately 102 for spheres that were several microns in diameter. The power dependence of the TPF intensity proved the second order nature of the interaction process involved.